Yayınlanmış 1 Ocak 2020
| Sürüm v1
Dergi makalesi
Açık
A new approach for affinity-based purification of horseradish peroxidase
Yazarlar/Oluşturanlar
- 1. Mus Alparslan Univ, Fac Sci & Literature, Mol Biol & Genet Dept, Mus, Turkey
- 2. Ibrahim Cecen Univ Agri, Vocat Sch Hlth Serv, Dept Med Serv & Tech, Agri, Turkey
- 3. Ataturk Univ, Sci Fac, Dept Chem, Erzurum, Turkey
Açıklama
We have developed efficient procedure for isolation of horseradish peroxidase (HRP) using aminobenzohydrazide-based affinity chromatography. Sepharose 4B-bounded aminobenzohydrazides are suitable for long-term use and large-scale purification. In this study, 26 aminobenzohydrazide derivatives were synthesized, characterized and defined as new HRP inhibitors. In addition, detailed inhibition effects of these molecules on HRP enzyme were investigated. Affinity matrix was formed by bonding aminobenzohydrazides, which exhibited inhibitory activity to sepharose-4B-l-tyrosine. HRP was isolated from crude homogenate in single step and purification factors were recorded as 1,151-fold (recovery of 8.5%) with 4-amino 3-bromo benzohydrazide and as 166.16-fold (recovery of 16.67 %) with 3-amino 4-chloro benzohydrazide.
Dosyalar
bib-f3bb4588-fe25-48be-b448-dc76a8b06daa.txt
Dosyalar
(212 Bytes)
| Ad | Boyut | Hepisini indir |
|---|---|---|
|
md5:6ba8139ab43b46bac16047f04679545b
|
212 Bytes | Ön İzleme İndir |