Yayınlanmış 1 Ocak 2012
| Sürüm v1
Dergi makalesi
Açık
Observing better transfection efficiency in primary cultured VSMCs: Comparison and development of two different protocols
Yazarlar/Oluşturanlar
- 1. Akdeniz Univ, Tip Fak, Tibbi Biyokimya Anabilim Dali, Sch Med, TR-07070 Antalya, Turkey
Açıklama
Aim: In this study, we have evaluated the procedure of transient transfection efficiency of vascular smooth muscle cells (VSMCs) with Lipofectamine (Life Technologies) and FuGENE (ROCHE, FuGENE HD Reagent) transfection reagents using the pCH110 eukaryotic assay vector containing the lacZ reporter gene. We also did try these attainments to transfect VSMCs with Ras(N17) DNA and affirmed our findings.
Dosyalar
bib-80c30d1b-baf0-497f-8e65-ae02bb02a7e5.txt
Dosyalar
(249 Bytes)
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md5:0384b2ef61ad2fa5871a2a5b2b05d09f
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249 Bytes | Ön İzleme İndir |