Yayınlanmış 1 Ocak 2012
| Sürüm v1
Dergi makalesi
Açık
Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus
Yazarlar/Oluşturanlar
- 1. Hosp Gen Mexico City, Epilepsy Clin, Mexico City, DF, Mexico
- 2. Hosp Gen Mexico City, Dept Pathol, Mexico City, DF, Mexico
- 3. Natl Med Ctr, Unit Med Res Neurol Dis, Mexico City, DF, Mexico
- 4. Ege Univ Bornova Izmir, Dept Biochem, Fac Pharm, Izmir, Turkey
- 5. Hungarian Acad Sci, Biol Res Ctr, H-6701 Szeged, Hungary
- 6. Natl Inst Neurol & Neurosurg Manuel Velasco Suare, Mexico City, DF, Mexico
- 7. Ctr Res & Adv Studies, Dept Pharmacobiol, Mexico City, DF, Mexico
Açıklama
Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%) Bmax values for the mesial TLE group, whereas the Kd values were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO-stimulated [35S]GTP?S binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations. (C) 2010 Wiley Periodicals, Inc.
Dosyalar
bib-8f593461-2788-4d61-9c3f-56b54d3b937e.txt
Dosyalar
(340 Bytes)
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